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The soft-clipped sequences that overhang the breakpoint are collapsed right into a consensus sequence employing a several sequence alignment (MSA) furnished during the seqAn library [27]. Wham applies three filters into the consensus sequences. Breakpoints aren't claimed in scenarios wherever consensus sequences are shorter than 10 bp or have a lot
Wham is created for paired-stop Illumina libraries with regular insert dimensions (~300bp-500bp). Wham integrates mate-pair mapping, split browse mapping, tender-clipping, substitute alignment and consensus sequence centered proof to forecast SV breakpoints with one-nucleotide precision. Wham generates a combined pileup (catalog of reads masking a
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